[HTML][HTML] An easy and sensitive method to profile the antibody specificities of HLA–specific memory B cells
GE Karahan, J Krop, C Wehmeier, YJH de Vaal… - …, 2019 - journals.lww.com
GE Karahan, J Krop, C Wehmeier, YJH de Vaal, J Langerak–Langerak, DL Roelen…
Transplantation, 2019•journals.lww.comBackground. Pretransplant immunological risk assessment is currently based on donor–
specific HLA antibodies in serum. Despite being an excellent source for antibodies
produced by bone marrow–residing plasma cells, serum analysis does not provide
information on the memory B–cell compartment. Although B–cell culture supernatants can
be used to detect memory B cell–derived HLA antibodies, low IgG concentrations can
preclude detectability of HLA antibodies in luminex single–antigen bead (SAB) assays …
specific HLA antibodies in serum. Despite being an excellent source for antibodies
produced by bone marrow–residing plasma cells, serum analysis does not provide
information on the memory B–cell compartment. Although B–cell culture supernatants can
be used to detect memory B cell–derived HLA antibodies, low IgG concentrations can
preclude detectability of HLA antibodies in luminex single–antigen bead (SAB) assays …
Abstract
Background.
Pretransplant immunological risk assessment is currently based on donor–specific HLA antibodies in serum. Despite being an excellent source for antibodies produced by bone marrow–residing plasma cells, serum analysis does not provide information on the memory B–cell compartment. Although B–cell culture supernatants can be used to detect memory B cell–derived HLA antibodies, low IgG concentrations can preclude detectability of HLA antibodies in luminex single–antigen bead (SAB) assays.
Methods.
Culture supernatants of polyclonally activated B cells from alloantigen exposed (n= 13) or nonexposed (n= 10) individuals were either concentrated 10–fold, or IgG was isolated by using a protein G affinity purification method to increase the IgG concentration. These processed culture supernatants, as well as paired serum samples were tested for the presence of HLA antibodies using luminex SAB analysis.
Results.
Lippincott Williams & Wilkins